This package provides everything you will need to begin sequencing specimens at your foray. Simply select the number of participants and the number of specimens you would like to have sequenced. 

The package includes:

One field data slip pad per participant (50 slips)
One instructional tri-fold per participant

One collection tube w/ buffer solution per sequenced specimen
One forward and one reverse read per specimen in our sequencing service
Sequence editing
Initial analysis of specimens via BLAST search
One tube box (holds 100 tubes)

Trifolds utilize the iNaturalist mobile applications for forays. Learn More

Shipping is included in the price. Return shipping not included. Call if you are looking for a combination that is not listed here. 

620.00
Number of Participants:

Number of Specimens:

These 2.0 mL screw-top microcentrifuge tubes contain 500 uL of Promega Nuclei Lysis Solution.

Each order contains 25, 50, or 100 tubes in a freezer box. Each order also contains field data slips.

Shipping is included in the product price. 

 

We encourage projects to collect tissue from fresh mushrooms as much as possible. This tissue will have the lowest chance for contamination and the highest quality DNA. It is fine to use dried tissue in these tubes, with the understanding that success rates may vary. (Typically dried tissue does fine as well, but drying conditions will have a significant effect on outcomes.)

25.00
Number of Tubes:

One way any mushroom hunter can contribute significant value to science is by saving collections of the mushrooms they encounter. The easiest way to organize these collections is by filling out a "field data slip" for each specimen you intend to save.  When participating in a foray or while out on a hike, simply take a picture of the slip with the specimen and store the slip with the sample. This allows the number on the slip to be recorded/associated with the images and it records the size of the specimen, as each slip has a ruler on the side. The numbers make organization easy when you are dealing with many specimens and images. It also ensures the most important data is preserved which increases the scientific value of your collections.

As you dry the specimen, remove the bottom portion of the slip and keep it with the specimen so they stay organized while they dry. Mushrooms will change their size, color, and shape as they dry, often making it impossible to sort out which species is which, unless there is some organization in the drying process. The bottom part of the slip provides you with an easy way to keep your specimens organized as they dry. 

Once your specimens are dry, put the upper portion of the data slip in a ziplock bag with the specimen. 

Each notepad contains 50 slips that are individually numbered. 

All prices include shipping to the United States. Contact us before ordering to Canada or Mexico.

 
Trifolds utilize the iNaturalist mobile applications for forays. Learn More

 

The field data slip flyer trifolds are available for download below if you would like to print them yourself. They are designed to be printed on 18" length paper.

DOWNLOAD: FLYER FRONT
DOWNLOAD: FLYER INSIDE

 

 

17.00
Number of Notepads:

Foray Trifold Included:

 

 

THIS PRODUCT CAN ONLY BE PURCHASED BY OFFICIAL PROJECTS OF THE NORTH AMERICAN MYCOFLORA PROJECT.

Any other purchases will be refunded.

For official partners of the North American Mycoflora Project, we offer a DNA sequencing service for macrofungal specimens at a highly discounted rate. Please read the information below fully before making a purchase. 

We may offer a rate lower than $10 per specimen for large projects. Please call or email to discuss your specific needs. 

Specimen Requirements

In order to get specimens sequenced as a part of this project, you must be a mushroom club or individual affiliated with the North American Mycoflora Project. All individual specimens must follow NAMP protocols in terms of documentation, metadata, and sample preparation. Minimum metadata requirements for a collection include a proposed species (or genus) name, collection date, collection location, and images of the specimen in a fresh condition. All specimens must be appropriately dried and individually packaged with herbarium labels. A spreadsheet containing the metadata of all included specimens needs to be sent to This email address is being protected from spambots. You need JavaScript enabled to view it.. You will be notified of the location to mail your specimens upon completion of your order.

Included Services

  1. DNA extraction (Specimen-dependent protocols)
  2. DNA amplification (PCR, with one additional repeat attempt)
  3. DNA sequencing (two reads - forward and reverse)
  4. Sequence editing
  5. GenBank submission


Timeframes

Expect sequences from specimens back in 1-2 months from the date they are received by the facility.

 

What to Expect Back

You will get two ABI sequence (raw data) files back per submitted specimen. One file will be for a forward read and one for the reverse read. Secondarily, you will also receive the edited sequence files. This comes in the form of FASTA files (special format that can be opened with a text editor) containing the final edited sequences that has been formed from the two reads. This is known as a “contig.” A download link for a zipped folder of these individual files will be provided.

Finally, you will receive a link to a project that contains the metadata of all of your specimens, as well as BLAST results of your specimens. Here you will be able to review the names that have been applied to the collections and can view the metadata that is associated with the specimen. This metadata will be used to upload your specimens to GenBank (a public DNA sequence repository).


By participating in this sequencing service, you agree to allow the mycoflora project to make your DNA sequences public after one year. You also agree to release the metadata of your sequences to other project members immediately. The sequences will be placed in our local BLAST search. Other users will have access to the observational records that appear in the BLAST results, but not the actual sequences.

 

Potential Risks

While genetically sequencing mushrooms is a process that is usually successful, not every specimen will be able to be successfully sequenced. There are a number of reasons this process can be unsuccessful. Following the mycoflora project protocols for collection and drying your specimens will give you the best chance for success. Potential risks at the sequencing facility include:

Unsuccessful PCR

DNA amplification may be inhibited by proteins, sugars, or other types of molecules that mushrooms contain. Two attempts will be made to successfully amplify a given sample. If not successful after two attempts, no further attempts will be made, and you will be given a 50% credit on future sequencing.

Low Quality Sequence

Occasionally, DNA extraction and amplification appear to be successful, but the sequence comes out muddled. This can be due to low quality DNA (old or poorly dried specimens), contaminants, or a number of other factors. If this occurs, these are sunk costs, and due to the low costs of this service, we cannot offer a refund or another sequencing attempt without additional cost.

We generally have success with than 90% of all samples submitted.

For best results, please only send specimens that were collected in the last 5 years. The older your specimens, the lower success rate you will receive. Older specimens can still be submitted after consultation with the project staff. Additional costs may apply.

 

 

200.00
Number of Specimens:

Tissue Submission Type:

About North American Mycoflora Project

We are working towards a single goal - the development of the first comprehensive mycoflora of North America. This project is a consortium of citizen scientists and professional mycologists performing a biological survey of all the macrofungi that occur in North America.

Latest News

22 February 2018
16 October 2017
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