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Frequently Asked Questions (FAQ)

General

What is a mycoflora, or funga?
A mycoflora, or more accurately a funga since fungi are not plants, is a survey of all of the fungi that exist in a defined geographic region. It could be as simple as a list of all of the species that occur within those boundaries. The more metadata that is associated with each species in the list, the more robust and useful your mycoflora will be. Essential metadata includes dates and locations where observations occured, and color images of specimens. Getting DNA sequence data from each of these collections will also add tremendous value to your project. And preserving dried physical collections of the specimens documented online will allow scientists to re-examine them in the future. Our protocols are designed to streamline organization of all of this data for your projects and to make DNA sequencing accessible and cost effective for your projects.
Who is operating the North American Mycoflora project?
What species are most important to this project?
What is a voucher specimen?

Participation

Can individuals participate in this project?
Yes, we do not require that you are a part of any formal organization to become a participating member or apply for funding. However, if there is a formal organization that covers your region, we ask that you work closely with them as a part of this project.
Why should I participate?

Funding

What type of funding is available?

We are currently not accepting new Grant applications, but hope to offer more grants in the future. See this page for details.

Do I have to be part of an organization to receive grants?

Protocols

Do I have options for where to store my data online?
We have data integrations with Mushroom Observer, iNaturalist, and MyCoPortal. 
Do I have to use the NAMP tissue collection tubes to participate?
Can tissue be taken after the specimen is dry?
Can we use Whatman cards for tissue collection?
How long can the collection tubes be stored?
Who keeps the voucher?

NAMP Field Data Slips

Do I have to use NAMP field data slips?
No. Field Data Slips are for your convenience in recording field data and organizing your photos and dried specimens. The unique identifier number for each specimen will be the number from iNaturalist or Mushroom Observer.

VOUCHERS:  Once your specimens are dry, put the main (top) portion of the Field Data Slip in a resealable bag with the specimen. After uploading your photos to iNaturalist of Mushroom Observer, add the unique iNat or MO number to the Field Data Slip.

SEQUENCING: When submitting dried tissue samples in vials for sequencing, use scotch tape to tape the unique iNat or MO number to the vial. The easiest method is to export the csv sheet from your Project (iNat) or List (MO) and print the numbers small enough that they can be cut and scotch-taped to a vial. We will not accept tubes with handwritten labels.

How are these slips intended to be used?
Does the entire slip need to be filled out for each specimen?
How many slips are in a notepad?
Does the order of the numbers matter in any way?
What if it is raining outside? What should I do then?

Sequence Analysis

What if my sequence comes back as a contaminant?
In a small number of cases, the sequence results that come back will represent some kind of contaminant, such as another type of fungi like a yeast or a mold. This is an unavoidable aspect of the process, and is much more common in certain groups, such as jelly fungi. It is also much more common for specimens that were not immediately placed in the dryer or were otherwise dried improperly. If you receive a sequence of a contaminant back, there is little we can do except attempt another DNA extraction from the orignal material. This would be an additional fee and there is no guarantee the next attempt would be successful. Typically, most researchers do not attempt this unless the specimen has some kind of unusual importance.

Follow the steps on this page if you encounter a contaminant in one of your sequences.

Other

I have a question I do not see answered. Who should I contact?
All general inquiries can be directed to info@mycoflora.org.
Can I use your logo?

About North American Mycoflora Project

NAMP is a 501(c)(3) non-profit organization working to create a continent-wide community of volunteer citizen scientists and professional mycologists to document the biodiversity of North American fungi.

©2017-2019 North American Mycoflora Project. All Rights Reserved.

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