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Paid Sequencing Service

Paid Sequencing Service

$266.00
Number of Specimens:

 THIS PRODUCT CAN ONLY BE PURCHASED BY REGISTERED PROJECTS OF THE NORTH AMERICAN MYCOFLORA PROJECT.  

Orders not from registered projects will be refunded.

Register here.

Please read the information below fully before making a purchase. 

Procedure

Review the DNA Sequencing Submission Checklist before proceeding.

When your specimens are ready to be mailed, SUBMIT THE SEQUENCING REQUEST here. 

This product is only for the sequencing service. It does not include tissue collection tubes: order tubes separately.

Specimen Requirements

In order to get specimens sequenced you have a registered project with the North American Mycoflora Project. All individual specimens must follow NAMP protocols in terms of documentation, metadata, and sample preparation. Minimum metadata requirements for a collection include a proposed species (or genus) name, collection date, collection location, and images of the specimen in a fresh condition. All specimens must be appropriately dried and individually packaged with herbarium labels. A spreadsheet containing the metadata of all included specimens must be included before sequencing will occur. You will be notified of the location to mail your specimens upon completion of your order.

Please see Quick Protocols and Detailed Project Protocols.

Pricing

$26.60 USD per specimen

PCR extraction & amplification: 14.00

Sequencing at Eurofin:  5.00

Sequence editing:  4.00

NAMP Overhead (15%):  3.60

Total:  26.60  

Additional merchant charge added at checkout if you order using PayPal or a credit card via PayPal: 2.2% plus $0.30 

This fee can be avoided by mailing a check to NAMP, 205 Three Oaks Drive, Athens, GA 30607 (Check “Money Order” at checkout).

NOTE: Rates are subject to change.

Included Services

  1. DNA extraction (Specimen-dependent protocols)
  2. DNA amplification (PCR, with one additional repeat attempt)
  3. DNA sequencing (two reads - forward and reverse)
  4. Sequence editing  
  5. Link to a project on MycoMap.com


Timeframes

We aim to get sequences from specimens within 2 months from the date they were received by the facility.

What to Expect Back

You will get two ABI sequence (raw data) files back per submitted specimen. One file will be for a forward read and one for the reverse read. Secondarily, you will also receive the edited sequence files. This comes in the form of FASTA files (special format that can be opened with a text editor) containing the final edited sequences that has been formed from the two reads. This is known as a “contig.” A download link for a zipped folder of these individual files will be provided.

Finally, you will receive a link to a project on MycoMap.com that contains the metadata of all of your specimens, as well as BLAST results of your specimens. Here you will be able to review the names that have been applied to the collections and can view the metadata that is associated with the specimen. This metadata will be used to upload your specimens to GenBank (a public DNA sequence repository).

By participating in this sequencing service, you agree to allow the mycoflora project to make your DNA sequences public after one year. You also agree to release the metadata of your sequences to other project members immediately. The sequences will be placed in our local BLAST search. Other users will have access to the observational records that appear in the BLAST results, but not the actual sequences.

Potential Risks

While genetically sequencing mushrooms is a process that is usually successful, not every specimen will be able to be successfully sequenced. There are a number of reasons this process can be unsuccessful. Following the mycoflora project protocols for collection and drying your specimens will give you the best chance for success.

For best results, please only send specimens that were collected in the last few years. The older your specimens, the lower success rate you will receive. Older specimens can still be submitted after consultation with the project staff. Additional costs may apply.

About North American Mycoflora Project

NAMP is a 501(c)(3) non-profit organization working to create a continent-wide community of volunteer citizen scientists and professional mycologists to document the biodiversity of North American fungi.

©2017-2019 North American Mycoflora Project. All Rights Reserved.

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