Quick Protocol Guide

Before your Project Begins

In the Field

  • Photograph specimens in the field with a smart phone (or other camera that collects time stamp and GPS information).
  • Collect specimens, keeping differnent collections separate. 

  • Fill out and photograph your Field Data Slip with each physically collection.  Note characteristics which are not captured photographically, and may be lost. E.g., taste, odor, staining. Note others which could be captured photographically, but often are not. E.g., nearest tree (at least hardwood vs conifer). Store this slip (or part of it with the unique number) with the specimen.


At Home Base

    • Take additional close-up photos of key features with a DSLR camera if desired.
    • Upload photos to a databased Internet platform like Mushroom Observer, iNaturalist, or MycoPortal, along with any notes.  .
      • Create a Species List in MO, or a Project in iNaturalist, and add observations you plan to collect tissue from for sequencing.
      • Include the NAMP field data slip number in MO "Herbarium ID" field or the iNaturalist "Voucher number(s)" field
      • NOTE: The observation number of the platform (with url) becomes the unique specimen ID -- for example, MO Observation 278460 or iNat Observation 6461208.  This ID will be used to coordinate between fungaria, MycoPortal, Genbank, etc. Facebook and Flicker are not acceptable platforms for the primary observational report for this project.
  • Triage and Tissue Collection
      • Determine which specimens are needed for vouchering and/or sequencing. This will vary depending on who is paying for sequencing.
      • Take tissue samples of specimens which are selected for sequencing.
  • Dry specimens
      • A food dehydrator is essential if you’re going to be processing a lot of specimens (drying temperature under 105 deg. F. is ideal).
      • Tear off the "drying" portion of the field data slip and tape it in the dehydrator with your specimen. 
      • Store specimens in a ziplock baggie with the remainder of the field data slip once they are cracker dry.

  • Print packing list and voucher labels.
    • Export observations in Species List (MO) or Project (iNat) as Mycoflora (MO) or Symbiota spreadsheet (csv). Ensure each record has a corresponding Mycoflora Specimen Number. 
    • Print a list of your specimens from the spreadsheet to serve as the packing list for your shipment to fungaria and the sequencing facility.
    • Ensure individual specimens are labeled with a species name, collection date, collection location, and specimen number. 
    • Print herbarium labels if requested by your partner fungarium.
    • If specimens are being sent for sequencing, email NAMP at submit@mycoflora.org about your shipment to get the address of the facility that will be sequencing your specimens. Include your Symbiota spreadsheet in this email.
  • Mail specimens to Fungarium and/or DNA Sequencing Lab
    • Place bags with labeled specimens in a small box along with the packing list. 
    • Mail your tissue samples to the lab doing sequencing. 
    • Email your fungarium about your upcoming shipment, along with the Symbiota upload spreadsheet. Mail your specimens to your fungarium.

About North American Mycoflora Project

We are working towards a single goal - the development of the first comprehensive mycoflora of North America. This project is a consortium of citizen scientists and professional mycologists performing a biological survey of all the macrofungi that occur in North America.

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16 October 2017
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