Sequencing Service

Sequencing Service

200.00
SKU : Sequencing_Service_116
Number of Specimens:

Tissue Submission Type:

 

 

THIS PRODUCT CAN ONLY BE PURCHASED BY OFFICIAL PROJECTS OF THE NORTH AMERICAN MYCOFLORA PROJECT.

Any other purchases will be refunded.

For official partners of the North American Mycoflora Project, we offer a DNA sequencing service for macrofungal specimens at a highly discounted rate. Please read the information below fully before making a purchase. This product DOES NOT INCLUDE SAMPLE TUBES. ONLY THE COST OF THE SEQUENCING. 

When your specimens are ready to be mailed, SUBMIT THE SEQUENCING REQUEST here. 

We may offer a rate lower than $10 per specimen for large projects. Please call or email to discuss your specific needs. 

Specimen Requirements

In order to get specimens sequenced as a part of this project, you must be a mushroom club or individual affiliated with the North American Mycoflora Project. All individual specimens must follow NAMP protocols in terms of documentation, metadata, and sample preparation. Minimum metadata requirements for a collection include a proposed species (or genus) name, collection date, collection location, and images of the specimen in a fresh condition. All specimens must be appropriately dried and individually packaged with herbarium labels. A spreadsheet containing the metadata of all included specimens must be included before sequencing will occur. You will be notified of the location to mail your specimens upon completion of your order.

Included Services

  1. DNA extraction (Specimen-dependent protocols)
  2. DNA amplification (PCR, with one additional repeat attempt)
  3. DNA sequencing (two reads - forward and reverse)
  4. Sequence editing
  5. GenBank submission


Timeframes

Expect sequences from specimens back in 1-2 months from the date they are received by the facility.

 

What to Expect Back

You will get two ABI sequence (raw data) files back per submitted specimen. One file will be for a forward read and one for the reverse read. Secondarily, you will also receive the edited sequence files. This comes in the form of FASTA files (special format that can be opened with a text editor) containing the final edited sequences that has been formed from the two reads. This is known as a “contig.” A download link for a zipped folder of these individual files will be provided.

Finally, you will receive a link to a project that contains the metadata of all of your specimens, as well as BLAST results of your specimens. Here you will be able to review the names that have been applied to the collections and can view the metadata that is associated with the specimen. This metadata will be used to upload your specimens to GenBank (a public DNA sequence repository).


By participating in this sequencing service, you agree to allow the mycoflora project to make your DNA sequences public after one year. You also agree to release the metadata of your sequences to other project members immediately. The sequences will be placed in our local BLAST search. Other users will have access to the observational records that appear in the BLAST results, but not the actual sequences.

 

Potential Risks

While genetically sequencing mushrooms is a process that is usually successful, not every specimen will be able to be successfully sequenced. There are a number of reasons this process can be unsuccessful. Following the mycoflora project protocols for collection and drying your specimens will give you the best chance for success. Potential risks at the sequencing facility include:

Unsuccessful PCR

DNA amplification may be inhibited by proteins, sugars, or other types of molecules that mushrooms contain. Two attempts will be made to successfully amplify a given sample. If not successful after two attempts, no further attempts will be made, and you will be given a 50% credit on future sequencing.

Low Quality Sequence

Occasionally, DNA extraction and amplification appear to be successful, but the sequence comes out muddled. This can be due to low quality DNA (old or poorly dried specimens), contaminants, or a number of other factors. If this occurs, these are sunk costs, and due to the low costs of this service, we cannot offer a refund or another sequencing attempt without additional cost.

We generally have success with than 80% of all samples submitted.

For best results, please only send specimens that were collected in the last 5 years. The older your specimens, the lower success rate you will receive. Older specimens can still be submitted after consultation with the project staff. Additional costs may apply.

 

 

About North American Mycoflora Project

NAMP is a 501(c)(3) non-profit organization. We are working towards a single goal - the development of the first comprehensive mycoflora of North America. This project is a consortium of citizen scientists and professional mycologists performing a biological survey of all the macrofungi that occur in North America.

Latest News

06 December 2018
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