Whole-plate Sequencing

Whole-plate Sequencing

600.00
SKU : Whole-plate_Sequencing_181
Sequence Editing:

GenBank Submission:

THIS PRODUCT CAN ONLY BE PURCHASED BY OFFICIAL PROJECTS OF THE NORTH AMERICAN MYCOFLORA PROJECT.

 

Any other purchases will be refunded.

For official partners of the North American Mycoflora Project, we offer a DNA sequencing service for macrofungal specimens at a highly discounted rate. Please read the information below fully before making a purchase. 

This product is being offered for projects that have the ability to conduct their own DNA extraction and PCR amplification. You should be comfortable in submitting PCR product for sequencing before purchasing this product.

YOU MAY ONLY SUBMIT WHOLE PLATES AT A TIME - 96 SAMPLES.
WE WILL ONLY DO SEQUENCING WITH BOTH FORWARD AND REVERSE READS. 
PRICE INCLUDES 192 TOTAL READS.

This can include any combination of primers, but you must provide a primer plate to go along with the sample plate if there is more than one primer combination on the plate. You must provide all materials for this product, and take full responsibility for the shipping to the facility and the results that are returned. There are NO OPTIONS to retry cells that do not produce results. Specifics on preparing a sequencing plate for submission.

All other sequencing service requirements remain in place for this product. In order to get specimens sequenced as a part of this project, you must be a mushroom club or individual affiliated with the North American Mycoflora Project. All individual specimens must follow NAMP protocols in terms of documentation, metadata, and sample preparation. Minimum metadata requirements for a collection include a proposed species (or genus) name, collection date, collection location, and images of the specimen in a fresh condition. All specimens must be appropriately dried and individually packaged with herbarium labels. A spreadsheet containing the metadata of all included specimens must be included on our Sequencing Submission Form. You will be notified of the location to mail your specimens upon completion of your order.

NOT included in this purchase:

  1. DNA extraction 
  2. DNA amplification 
  3. 3 mM sequencing primers
  4. Lab consumables

Included in this purchase:

DNA sequencing (two reads - forward and reverse)
PCR Cleanup

Optional services for this purchase:

     1. Sequence editing
     2. GenBank submission


Timeframes

Expect sequences from specimens back in 2-3 weeks from the time they arrive at the facility.

 

What to Expect Back

You will get two ABI sequence (raw data) files back per submitted PCR product. One file will be for a forward read and one for the reverse read. 




By participating in this sequencing service, you agree to allow the mycoflora project to make your DNA sequences public after one year. You also agree to release the metadata of your sequences to other project members immediately. The sequences will be placed in our local BLAST search. Other users will have access to the observational records that appear in the BLAST results, but not the actual sequences.

About North American Mycoflora Project

NAMP is a 501(c)(3) non-profit organization. We are working towards a single goal - the development of the first comprehensive mycoflora of North America. This project is a consortium of citizen scientists and professional mycologists performing a biological survey of all the macrofungi that occur in North America.

©2017 North American Mycoflora Project. All Rights Reserved.

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